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autosomal recessive polycystic kidney disease (ARPKD)


Here is the microscopic appearance of autosomal recessive polycystic kidney disease (ARPKD). Note that the cysts fill most of the parenchyma, and it is hard to find glomeruli. Many of the cysts are elongated and radially arranged from the center of the kidney on the right, much like spokes on a wagon wheel   
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necrotic hepatocytes


If the passive congestion is pronounced, then there can be centrilobular necrosis, because the oxygenation in zone 3 of the hepatic lobule is not great. The light brown pigment seen here in the necrotic hepatocytes around the central vein is lipochrome   
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Cystine Crystals


6 sided Does not have to be equal Edges are sharp   
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RETICULOCYTE


Size of the cell: 8 - 12 mm Shape of the cell: round Colour of cytoplasm: as in mature erythrocytes, with violet tint Granularity: none, very rarely with basophilic stippling Nucleus' shape: none Occurrence in blood: 0.5 - 1.5 % of erythrocytes Comment: The indicated cell is the reticulocyte, which in the May-Grunwald-Giemsa staining differs from the remaining mature erythrocytes. The indicated cell is greater, the perinuclear zone is not present and takes polychromatophilic staining. Staining: MGG Magnification: x 1000    
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Red blood cells


Red blood cells in urine appear as refractile disks. With hypertonicity of the urine, the RBC's begin to have a crenated appearance   
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cryptosporidia


Cryptosporidia are seen along the brush border of the intestinal mucosa in this electron micrograph   
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Sepedonium 40x


Sepedonium   
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C.albicans 10x


C. albicans    
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Calcium Oxalate Monohydrate


Calcium oxalate monohydrate crystals vary in size and may have a spindle, oval, or dumbbell shape. Most commonly, they appear as flat, elongated, six-sided crystals ("fence pickets") such as shown to the right. The arrow in the photo indicates a "daughter" crystal forming on the face of a larger underlying crystal. We have not observed this form of calcium oxalate crystals in urine of normal dogs and cats, and believe their presence is virtually always associated with ethylene glycol intoxication.   
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The tubular vacuolization and tubular dilation


270) The tubular vacuolization and tubular dilation here is a result of the toxic effect of ethylene glycol poisoning. This is representative of acute tubular necrosis (ATN), which has many causes. ATN resulting from toxins usually has diffuse tubular involvement, whereas ATN resulting from ischemia (as in profound hypotension from cardiac failure) has patchy tubular involvement   
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RBC Cast


Microscopic urinalysis reveals a protein cast containing red blood cells. The RBC's are anucleate. The cast takes the shape of the renal tubule Significance: Glomerular disease , Common conditions:Acute glomerulo nephritis, Lupus nephritis, Goodpasture's syndrome ,Malignant hypertension .   
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Cryptococcus neoformans


Cryptococcus neoformans often produces a pneumonia, because infection is acquired via the respiratory tract. Here, C neoformans organisms have a clear capsule surrounding the pale blue nucleus with H and E staining   
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SICKLE CELL


Definition: Elongated erythrocytes, usually curved with sharply pointed one or two poles. Occurrence in blood: normally not present Comment: Single sickle cell. Also distinct anisopoikilocytosis and staining disturbance of erythrocytes. Staining: MGG Magnification: x 1000    
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Dientamoeba fragilis 100x


Dientamoeba fragilis    
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viral hepatitis and councilman body


Individual hepatocytes are affected by viral hepatitis. Viral hepatitis A rarely leads to signficant necrosis, but hepatitis B can result in a fulminant hepatitis with extensive necrosis. A large pink cell undergoing "ballooning degeneration" is seen below the right arrow. At a later stage, a dying hepatocyte is seen shrinking down to form an eosinophilic "councilman body" below the arrow on the left   
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Alcaligenes faecalis


Small, grey, smooth colonies which cause no alteration of the blood. The culture has been incubated at 30°C. General characteristics: Gram-negative. Catalase- and oxidase-positive. Motile. Obligately aerobic. Does not attack sugars. As non-fermentative Gram-negative rods, they are art of the psychrotropic spoilage flora of raw foods. Optimum temperature for growth is 20 - 37°C    
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SCHISTOCYTES


Definition: Irregularly shaped erythrocyte. Occurrence in blood: normally not present Comment: The indicated schisocyte is one of six presented in the picture. Also discrete anisocytosis of the erythrocytes. Staining: MGG Magnification: x 1000 1. schistocyte 2. microcyte    
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Uric acid crystal


Rhomboid forms of uric acid dyhydrate   
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Phase contrast of Corynebacterium pseudotuberculosis


Small, irregular rods, some of them club formed. Non-motile   
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CMV


Immunoperoxidase staining may reveal CMV in some cases where the inclusions are not numerous or characteristic   
 

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